What is NAD+?
Nicotinamide adenine dinucleotide (NAD+) is an essential cofactor found in all living cells and belongs to the class of nucleotide cofactors. This dinucleotide consists of two nucleoside molecules linked by their phosphate groups, comprising nicotinamide riboside and adenosine. NAD+ was first discovered in 1906 by British biochemists Arthur Harden and William John Young during their research into alcoholic fermentation in yeast cells. The molecule plays a crucial role as an electron acceptor in redox reactions and is fundamental for cellular energy production.
Mechanism of Action
NAD+ functions as a critical cofactor for over 400 enzymatic reactions in the human body. The molecule primarily acts via three main pathways: as a cofactor for dehydrogenases in glycolysis and the citric acid cycle, as a substrate for sirtuins (SIRT1-7) involved in gene expression and DNA repair, and as a substrate for poly(ADP-ribose) polymerases (PARPs) that detect and repair DNA damage. NAD+ accepts electrons and is reduced to NADH, generating energy for ATP synthesis. Additionally, NAD+ activates sirtuin proteins by cleaving off NAM (nicotinamide), resulting in deacetylation of histones and transcription factors, leading to improved mitochondrial biogenesis and cellular stress resistance.
Research Results
Preclinical studies have shown promising results for NAD+ supplementation. A landmark study published in Cell (2016) by Yoshino et al. demonstrated that NAD+ precursors increased the lifespan of mice by 10-15% and improved mitochondrial function. In C. elegans models, NAD+ supplementation resulted in a 25% extension of lifespan (Mouchiroud et al., Nature 2013). Cell culture studies show that increased NAD+ levels enhance SIRT1 activity by 40-60%, leading to improved DNA repair and reduced oxidative stress markers. Research on yeast cells showed a 30% improvement in mitochondrial respiration and ATP production when treated with NAD+ precursors (Belenky et al., Cell 2007).
Available Variants and Purity
Our NAD+ is available in two research variants: 500mg (€65) and 1000mg (€119), both produced according to pharmaceutical GMP standards. The product has a guaranteed purity of ≥99.9% as verified by high-resolution LC-MS/MS analysis. Each batch is independently tested by a certified analytical laboratory for purity, identity, and microbiological contaminants. A Certificate of Analysis (CoA) is provided with every order, detailing specifications and test results.
Storage and Handling
Store NAD+ powder at -20°C in the original sealed container, protected from light and moisture. Under these conditions, the product retains its stability for at least 24 months. For reconstitution, sterile water or phosphate-buffered saline (PBS, pH 7.4) is recommended. Reconstituted solutions should be used within 48 hours when stored at 4°C, or can be frozen at -80°C for long-term storage up to 6 months. Avoid repeated freeze-thaw cycles to prevent degradation.
Research Disclaimer
This NAD+ product is intended for in vitro scientific research by qualified researchers and laboratories only. It is not approved for human consumption, therapeutic application, or diagnostic purposes. Users are responsible for complying with local regulations regarding the handling of research chemicals. Dutch Peptide Labs disclaims all responsibility for misuse of this product outside scientific research contexts.
